Year: 2021 | Month: June | Volume 11 | Issue 3
Polymorphism of Deleted in Azoospermia-Like (DAZL) Gene in Ongole, Crossbred and Murrah Bulls used for Artificial Insemination in Andhra Pradesh, India
Sudhakar Krovvidi
Vinoo
R.
Muralidhar Metta
Deepthi
Ch.
Surendra
K
Jayamaduri
B
DOI:10.30954/2277-940X.03.2021.6
Abstract:
Selection of elite crossbred bulls at young age is inevitable for uninterrupted supply of semen to vast bovine population for genetic improvement and production. Identification of sperm molecular biomarkers could be a better approach for selection of young bull for efficient use of resources. The present study was undertaken on 270 bulls of various genetic groups to assess the polymorphism at two loci of DAZL gene by PCR-RFLP. On restriction fragment analysis of 3’UTR/DedI polymorphism was observed in Ongole, Jersey, Jersey crossbred, Holstein, and Murrah bulls. HF crossbred bulls were monomorhic with fixation of A allele and CC genotype was evident only in Ongole bulls. In the bulls across the breeds studied the AA genotype was predominant (90.0 to 99.0). PCR-RFLP of region comprising missense variant in exon 3 was performed with BstY1 and the study revealed that all the bulls were monomorphic at the loci. Further studies to identify polymorphisms and evaluating their association might elucidate the role of DAZL SNPs as a marker for fertility traits in bulls.
Highlights
- DAZL gene polymorphism using PCR-RFLP was studied in 270 bulls.
- Polymorphism at 3’UTR/DedI was observed in all the breeds except in HF crossbred bulls.
- Restriction digestion of missense variant in exon 3 with BstY1 revealed all bulls are monomorphic.
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